Adaptation to serum-free culture of HEK 293T and Huh7.0 cells

Adaptation to serum-free culture of HEK 293T and Huh7.0 cells

Background The fetal bovine serum (FBS) is a component of higher value added to the medium, which may cause difficulty in the process of recovery and purification of bioproduct. Considering all these reasons, it is essential to develop a simple cell adaptation process for the culture of cells in serum-free, animal component-free, or protein-free medium conditions [1,2]. The adaptation of cellul...

Adaptation of glucocerebrosidase-producing CHO cells to serum-free suspension culture

Background Deficiency of the lysosomal glucocerebrosidase (GCR) enzyme results in Gaucher disease, the most common inherited storage disorder [1]. Current treatment consists of enzyme replacement therapy by administration of exogenous GCR. Although effective, it is exceptionally expensive [2]. In Brazil, the public healthcare system provides the drug free of charge for all Gaucher’s patients, w...

Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII

BACKGROUND Hemophilia A is a bleeding disorder caused by deficiency in coagulation factor VIII. Recombinant factor VIII (rFVIII) is an alternative to plasma-derived FVIII for the treatment of hemophilia A. However, commercial manufacturing of rFVIII products is inefficient and costly and is associated to high prices and product shortage, even in economically privileged countries. This situation...

Lentiviral Vectors Mediate Long-Term and High Efficiency Transgene Expression in HEK 293T cells

OBJECTIVES Lentiviral vectors have been used successfully to rapidly produce decigram quantities of active recombinant proteins in mammalian cell lines. To optimize the protein production platform, the roles of Ubiquitous Chromatin Opening Element (UCOE), an insulator, and selected promoters were evaluated based on efficiency and stability of foreign gene expression mediated by lentiviral vecto...

Adaptation of Horsesickness Virus to Tissue Culture